Uppsala-Sweden – Expand the Coliroids into Colorful Light Sensing
A bacterial photocopy machine? They must be out of their mind. It must have something to do with light sensors and color expression. Judging by the Uppsala teams colorful t-shirts they sure know what they are talking about!
They presentation introduction is like a ceremony taking place. The presenter introduces the topic and principle goals as if he is announcing a dramatic news item. It is clear and crisps what the Uppsala team wants to achieve.
A blue light sensor (YF1) is shown on the screen after a rapid animation that triggers a mild laugh in the room. The blue light sensor is a new addition to the already existing red and green light sensors in the Registry. All three sensors are proposed to be transformed into the same cell, coupled to their own expression cassette for the production of the associated color.
All together the team wanted to insert a great amount of basepairs they chose to incorporate the genes into the bacterial genome. For this they used the Lamba red assembly system. Despite a nice full overview of the technique, this approach has not been fully characterized yet. Future teams can pick up this task, because the Lamba red plasmid is now available in the Registry.
Btw, contrarily to using the red light sensor from the registry, the team got the sensor from the authors of the original paper. They succesfully removed the illegal restriction sites as well. So, for those of you ever planning to use this sensor: please contact Uppsala.
Summarizing, it was a colorful presentation with an interesting Q&A session afterwards. A bacterial photocopier still seems to be a bridge to far, but to say the least this is a proper foundation.
http://2011.igem.org/Regions/Europe/Jamboree/Blog
They presentation introduction is like a ceremony taking place. The presenter introduces the topic and principle goals as if he is announcing a dramatic news item. It is clear and crisps what the Uppsala team wants to achieve.
A blue light sensor (YF1) is shown on the screen after a rapid animation that triggers a mild laugh in the room. The blue light sensor is a new addition to the already existing red and green light sensors in the Registry. All three sensors are proposed to be transformed into the same cell, coupled to their own expression cassette for the production of the associated color.
All together the team wanted to insert a great amount of basepairs they chose to incorporate the genes into the bacterial genome. For this they used the Lamba red assembly system. Despite a nice full overview of the technique, this approach has not been fully characterized yet. Future teams can pick up this task, because the Lamba red plasmid is now available in the Registry.
Btw, contrarily to using the red light sensor from the registry, the team got the sensor from the authors of the original paper. They succesfully removed the illegal restriction sites as well. So, for those of you ever planning to use this sensor: please contact Uppsala.
Summarizing, it was a colorful presentation with an interesting Q&A session afterwards. A bacterial photocopier still seems to be a bridge to far, but to say the least this is a proper foundation.
http://2011.igem.org/Regions/Europe/Jamboree/Blog
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